Goal: Assemble simple regulatory parts that control a reporter (fluorescence). Tune parameters, then simulate to see output and dose-response curves. This is a safe, conceptual simulator for education—no wet-lab steps.
- Drag blocks from the palette into the 6 left-to-right slots. You can mix Basal promoters, Activators/Repressors (A or B), logical AND/OR with A or B, NOT, Amplifier, and a Reporter (one per circuit).
- Inputs A & B: Use the sliders to set inducer concentrations (0–100 μM). Activators use Hill activation; Repressors apply Hill-style repression.
- Tune parameters: Click a placed block → adjust Kd, Hill coefficient n, and strength. For combiner gates, pick whether they combine with A or B.
- Run: Press Run to animate flow and compute the reporter output. Use Plot Dose Response to sweep A or B and see a curve.
- Presets: Quickly load typical behaviors like AND, OR, NAND, A AND NOT B.
Math (simplified): Activation: \( H_{act}(x) = \frac{x^n}{K^n + x^n} \). Repression factor: \( H_{rep}(x) = \frac{1}{1 + (x/K)^n} \). AND ≈ multiply signals. OR ≈ \(1 - (1-a)(1-b)\). Amplifier multiplies by a gain (clamped 0..1).
Safety: Educational visualization only; not a design pipeline and not medical or lab guidance.